ABSTRACT
Aim: Our goal was to identify the putative association of rs4072111 variant in IL-16 gene and HCV susceptibility in an Iranian population
Background: Interleukin 16 [IL-16], a multifunctional cytokine, plays a vital role in modulation of immune system
Methods: In present case control and cross sectional study, IL-16 gene variant in 300 patients with hepatitis C [HCV] infection and 300 healthy individuals were analyzed. To evaluate this possible association, genomic DNA from venous blood was extracted and genotypes of IL-16 rs4072111 variant were determined by polymerase chain reaction- Fragments Length Polymorphism Technique [PCR-RFLP]. Then, rs4072111 C/T genotypes frequency and allelic distribution were evaluated in each group
Results: The results of genotyping showed 82% CC, 17.3% CT, 0.7% TT in the control group and 78% CC, 20% CT and 2% TT in the case group. The distribution of rs4072111 C allele was 90.7% in controls and 88% in case group respectively. However, no correlation between IL-16 rs4072111 C/T variants and susceptibility to chronic HCV infection was found in the present study
Conclusion: We concluded the rs4072111 C/T cannot be considered as a proper biomarker to identify susceptibility to chronic hepatitis C virus infection
ABSTRACT
This study investigated the role of CD86 +237 G/C polymorphism in intensifying the risk of CRC development. Colorectal cancer [CRC] is a multi-factorial diseases. Genetic background could affect the susceptibility of individuals to CRC development. CD86 is a co-stimulatory factor on antigen-presenting cells that plays key roles in several cancer related mechanisms such as autoimmunity, transplantation and tumor immunity. A total of 300 individuals, 150 known CRC patients and 150 healthy control individuals, were subjected for the study. CD86 rs17281995 single nucleotide polymorphism [SNP] was genotyped using Allelic Discrimination method. A statistically significant difference was found among CD86 gene polymorphism [rs17281995] and risk of CRC development. The frequency of GG, GC and CC in control subjects was determined as 38%, 57.3% and 4.7% respectively and in CRC subjects were determined as 42%, 85% and 23% respectively. The data shows a significant association between CC genotype [P=0.007] and C allele [P=0.017] of the studied polymorphism and risk of CRC. CC genotype and C allele are also more frequent in female patients when the data is stratified according to gender status. Our results suggest that CD86 gene alteration could affect the individual's risk for developing CRC among Iranian population and could be used as an important prognostic factor associated with risk of CRC